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mouse thorase  (Addgene inc)


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    Structured Review

    Addgene inc mouse thorase
    (A) Domain diagram of <t>Mouse</t> <t>Thorase.</t> The construct used in this study comprised residues 41-361. (B) Size exclusion chromatography elution profile of Thorase Monomer. (C and D) Preparation of Thorase filaments by incubation with non-hydrolysable ATP Analog (ATP-γ-S) (E) Negative stain electron micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments (black arrows) are readily distinguished (F) Cryo-EM micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments in vitreous ice (black arrows) are readily distinguished.
    Mouse Thorase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse thorase/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    mouse thorase - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Cryo-EM Structure of AAA + ATPase Thorase Reveals Novel Helical Filament Formation"

    Article Title: Cryo-EM Structure of AAA + ATPase Thorase Reveals Novel Helical Filament Formation

    Journal: bioRxiv

    doi: 10.1101/2024.11.22.624887

    (A) Domain diagram of Mouse Thorase. The construct used in this study comprised residues 41-361. (B) Size exclusion chromatography elution profile of Thorase Monomer. (C and D) Preparation of Thorase filaments by incubation with non-hydrolysable ATP Analog (ATP-γ-S) (E) Negative stain electron micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments (black arrows) are readily distinguished (F) Cryo-EM micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments in vitreous ice (black arrows) are readily distinguished.
    Figure Legend Snippet: (A) Domain diagram of Mouse Thorase. The construct used in this study comprised residues 41-361. (B) Size exclusion chromatography elution profile of Thorase Monomer. (C and D) Preparation of Thorase filaments by incubation with non-hydrolysable ATP Analog (ATP-γ-S) (E) Negative stain electron micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments (black arrows) are readily distinguished (F) Cryo-EM micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments in vitreous ice (black arrows) are readily distinguished.

    Techniques Used: Construct, Size-exclusion Chromatography, Incubation, Staining, Cryo-EM Sample Prep



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    mouse thorase  (Addgene inc)
    93
    Addgene inc mouse thorase
    (A) Domain diagram of <t>Mouse</t> <t>Thorase.</t> The construct used in this study comprised residues 41-361. (B) Size exclusion chromatography elution profile of Thorase Monomer. (C and D) Preparation of Thorase filaments by incubation with non-hydrolysable ATP Analog (ATP-γ-S) (E) Negative stain electron micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments (black arrows) are readily distinguished (F) Cryo-EM micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments in vitreous ice (black arrows) are readily distinguished.
    Mouse Thorase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse thorase/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    mouse thorase - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Domain diagram of Mouse Thorase. The construct used in this study comprised residues 41-361. (B) Size exclusion chromatography elution profile of Thorase Monomer. (C and D) Preparation of Thorase filaments by incubation with non-hydrolysable ATP Analog (ATP-γ-S) (E) Negative stain electron micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments (black arrows) are readily distinguished (F) Cryo-EM micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments in vitreous ice (black arrows) are readily distinguished.

    Journal: bioRxiv

    Article Title: Cryo-EM Structure of AAA + ATPase Thorase Reveals Novel Helical Filament Formation

    doi: 10.1101/2024.11.22.624887

    Figure Lengend Snippet: (A) Domain diagram of Mouse Thorase. The construct used in this study comprised residues 41-361. (B) Size exclusion chromatography elution profile of Thorase Monomer. (C and D) Preparation of Thorase filaments by incubation with non-hydrolysable ATP Analog (ATP-γ-S) (E) Negative stain electron micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments (black arrows) are readily distinguished (F) Cryo-EM micrograph of the Thorase in running buffer with the addition of 1.0 mM ATP-γ-S in which filaments in vitreous ice (black arrows) are readily distinguished.

    Article Snippet: Mouse Thorase (residues 41–361), N-terminally fused with a human rhinovirus 3C protease cleavable GST tag, was cloned into an ampicillin resistant pGEX-6P-1 vector (addgene).

    Techniques: Construct, Size-exclusion Chromatography, Incubation, Staining, Cryo-EM Sample Prep